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Tuning Enzyme Activity for Nonaqueous Solvents: Engineering an Enantioselective "Michaelase" for Catalysis in High Concentrations of Ethanol.

Chembiochem : a European journal of chemical biology - 15 May 2020

Guo Chao, Biewenga Lieuwe, Lubberink Max, van Merkerk Ronald, Poelarends Gerrit J

Abstract excerpt

Enzymes have evolved to function under aqueous conditions and may not exhibit features essential for biocatalytic application, such as the ability to function in high concentrations of an organic solvent. Consequently, protein engineering is often required to tune an enzyme for catalysis in non-aqueous solvents. In this study, we have used a collection of nearly all single mutants of 4-oxalocrotonate tautomerase,...

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Identifiers

PMID: 31886617
DOI: 10.1002/cbic.201900721
PMCID: PMC7317446

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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