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Article

Mutation choice to eliminate buried free cysteines in protein therapeutics.

Journal of pharmaceutical sciences - 1 Feb 2015

Xia Xue, Longo Liam M, Blaber Michael

Abstract excerpt

Buried free-cysteine (Cys) residues can contribute to an irreversible unfolding pathway that promotes protein aggregation, increases immunogenic potential, and significantly reduces protein functional half-life. Consequently, mutation of buried free-Cys residues can result in significant improvement in the storage, reconstitution, and pharmacokinetic properties of protein-based therapeutics. Mutational design to...

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PMID: 25312595
DOI: 10.1002/jps.24188

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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