Article

An improved and readily available version of <i>Bst</i> DNA Polymerase for LAMP, and applications to COVID-19 diagnostics

2020-10-05

Abstract excerpt

Despite the fact that strand-displacing activity is of great utility for a variety of applications, including isothermal amplification assays, there are relatively few strand-displacing DNA polymerases. In particular, the thermotolerant DNA polymerase from Geobacillus stearothermophilus ( previously Bacillus stearothermophilus), Bst DNA polymerase ( Bst DNAP), is used in a variety of assays, including loop-mediate...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 13363621-82a4-5754-8f61-ce69fa88d76f
DOI: 10.1101/2020.10.02.20203356

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

An improved and readily available version of <i>Bst</i> DNA Polymerase for LAMP, and applications to COVID-19 diagnosticsDOI 10.1101/2020.10.02.20203356Read

Enhancement of Polymerase Activity of the Large Fragment in DNA Polymerase I from Geobacillus stearothermophilus by Site-Directed Mutagenesis at the Active Site.Semantic proximity Read

A Novel Linear Displacement Isothermal Amplification with Strand Displacement Probes (LDIA-SD) in a Pocket-Size Device for Point-of-Care Testing Of Infectious DiseasesSemantic proximity Read

3’ end hydrolysis activity of the Bst polymerase enables rapid and sensitive identification of SARS-CoV-2 variantsSemantic proximity Read

Circular permutation engineering improves amplification efficiency of Bst DNA polymerase.Semantic proximity Read

Robust strand exchange reactions for the sequence-specific, real-time detection of nucleic acid amplicons.Semantic proximity Read

Fluorometric DNA Polymerase Activity Assay for Resource-Limited Enzyme ManufacturingSemantic proximity Read

A high-specificity flap probe-based isothermal nucleic acid amplification method based on recombinant FEN1-Bst DNA polymeraseSemantic proximity Read

qByte: Open-source isothermal fluorimeter for democratizing analysis of nucleic acids, proteins and cellsSemantic proximity Read

A novel reporter system for temperature dependent analysis of nucleic acid amplification testsSemantic proximity Read

The attachment of Sso7d-like protein improves processivity and resistance to inhibitors of M-MuLV reverse transcriptaseSemantic proximity Read

Toehold-controlled ligation and transcription for accurate COVID-19 genotyping.Semantic proximity Read

Engineering of DNA polymerase I from Thermus thermophilus using compartmentalized self-replication.Semantic proximity Read