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Article

Computational design of a thermolabile uracil-DNA glycosylase of Escherichia coli.

Biophysical journal - 5 Apr 2022

Park Seongjun, Shin Yong-Keol, Yoon Jeong-Yeon, Nam Ki-Hoon, Munashingha Palinda Ruvan, Park Soyeong, Park So-Yeon, Kim Sangyeol, Lee Juhwan, Seo Min Jae, Yu Wookyung, Seo Yeon-Soo, Chang Iksoo

Abstract excerpt

Polymerase chain reaction (PCR) is a powerful tool to diagnose infectious diseases. Uracil DNA glycosylase (UDG) is broadly used to remove carryover contamination in PCR. However, UDG can contribute to false negative results when not inactivated completely, leading to DNA degradation during the amplification step. In this study, we designed novel thermolabile UDG derivatives by supercomputing molecular dynamic...

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Identifiers

PMID: 35183522
DOI: 10.1016/j.bpj.2022.02.027
PMCID: PMC9034189

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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