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Article

Rapid microfluidic sample preparation for mass spectrometric analysis of wild-type and mutant BRAF protein.

Analytica chimica acta - 15 Mar 2026

Lin Yen-Heng, Chang Heng-Yun, Wu Chia-Chun, Hsiao Yung-Chin, Wen Ying-Hao, Yu Jau-Song

Abstract excerpt

BACKGROUND: The BRAF V600E mutant protein is a valuable biomarker for the diagnosis and prognosis of colorectal cancer. Quantitative detection by mass spectrometry (MS) requires purification from complex cell extracts and digestion into surrogate peptides, a process that traditionally takes at least ∼12 h, which is time-consuming and labor-intensive, limiting clinical applicability. RESULTS: We present an...

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PMID: 41663222
DOI: 10.1016/j.aca.2026.345146

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BRAF

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

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