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Article

HistoMosaic Detecting KRAS G12V Mutation Across Colorectal Cancer Tissue Slices through in Situ PCR.

Analytical chemistry - 1 Mar 2016

Raub Christopher B, Lee Chen-Chung, Shibata Darryl, Taylor Clive, Kartalov Emil

Abstract excerpt

We report on HistoMosaic, a novel technique for genetic analysis of formalin-fixed, paraffin-embedded tissue slices. It combines microfluidic compartmentalization, in situ allele-specific PCR, and fluorescence microscopy. The experimental proof of principle was achieved by in situ detection of KRAS G12V mutation in colorectal cancer tissues and is presented herein. HistoMosaic offers the ability to detect...

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Identifiers

PMID: 26820161
DOI: 10.1021/acs.analchem.5b04460
PMCID: PMC7446286

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KRAS

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