Article
Two-step protein labeling utilizing lipoic acid ligase and Sonogashira cross-coupling.
Bioconjugate chemistry - 17 Sept 2014
Hauke Sebastian, Best Marcel, Schmidt Tobias T, Baalmann Mathis, Krause André, Wombacher Richard
Abstract excerpt
Labeling proteins in their natural settings with fluorescent proteins or protein tags often leads to problems. Despite the high specificity, these methods influence the natural functions due to the rather large size of the proteins used. Here we present a two-step labeling procedure for the attachment of various fluorescent probes to a small peptide sequence (13 amino acids) using enzyme-mediated peptide labeling...
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