Article
Efficient proximity labeling in living cells and organisms with TurboID.
Nature biotechnology - 1 Oct 2018
Branon Tess C, Bosch Justin A, Sanchez Ariana D, Udeshi Namrata D, Svinkina Tanya, Carr Steven A, Feldman Jessica L, Perrimon Norbert, Ting Alice Y
Abstract excerpt
Protein interaction networks and protein compartmentalization underlie all signaling and regulatory processes in cells. Enzyme-catalyzed proximity labeling (PL) has emerged as a new approach to study the spatial and interaction characteristics of proteins in living cells. However, current PL methods require over 18 h of labeling time or utilize chemicals with limited cell permeability or high toxicity. We used...
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