Article
Directed molecular evolution reveals Gaussia luciferase variants with enhanced light output stability.
Analytical chemistry - 5 Mar 2013
Degeling M Hannah, Bovenberg M Sarah S, Lewandrowski Grant K, de Gooijer Mark C, Vleggeert-Lankamp Carmen L A, Tannous Marie, Maguire Casey A, Tannous Bakhos A
Abstract excerpt
Gaussia Luciferase (Gluc) has proven to be a powerful mammalian cell reporter for monitoring numerous biological processes in immunology, virology, oncology, and neuroscience. Current limitations of Gluc as a reporter include its emission of blue light, which is absorbed by mammalian tissues, limiting its use in vivo, and a flash-type bioluminescence reaction, making it unsuited for high-throughput applications....
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