Substrate specificity of Burkholderia pseudomallei multidrug transporters is influenced by the hydrophilic patch in the substrate-binding pocket.

Abstract excerpt

The Gram-negative pathogen Burkholderia pseudomallei possesses multiple resistance-nodulation-division superfamily transporters that contribute to multidrug resistance, including BpeB and BpeF. Structural studies of BpeB and BpeF have identified a hydrophilic patch in their substrate-binding pocket. To investigate the relationship between this hydrophilic patch and substrate specificity, mutant analyses were...

Topics

• Substrate Specificity
• Burkholderia pseudomallei
• Binding Sites
• Bacterial Proteins
• Hydrophobic and Hydrophilic Interactions
• Drug Resistance, Multiple, Bacterial
• Anti-Bacterial Agents
• Escherichia coli
• Mutation
• Membrane Transport Proteins
• Microbial Sensitivity Tests
• Aminoglycosides