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Article

Impact of humanization scaffold design on the functional activity of FGFR2-targeting chicken scFvs.

Journal of biotechnology - 1 Jan 2026

Yii Shyi-Chun, Lin Tsai-Yu, Chang Fu-Ling, Jaw Fu-Shan, Tsai Keng-Chang, Lin Yun-Shih, Lee Yu-Ching

Abstract excerpt

Fibroblast growth factor receptor 2 (FGFR2) is frequently overexpressed in gastric cancer and represents a promising therapeutic target. We developed FGFR2-specific single-chain variable fragments (scFvs) using a chicken-derived immune library and a refined cell-based panning strategy incorporating FGFR2-knockdown cells for negative selection. The lead clone, scFv R21, exhibited high binding affinity and strong...

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Share to Antibodies, Monoclonal, Humanized
Share to Chickens

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Gene Knockdown Techniques

Share to Immunoglobulin G

Share to Receptor, Fibroblast Growth Factor, Type 2

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Share to Stomach Neoplasms

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Source

Identifiers

PMID: 41110811
DOI: 10.1016/j.jbiotec.2025.10.005

Genes

FGFR2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 8 Sept 2026.

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