Utargetome: A targetome prediction tool for modified U1-snRNAs to identify distal-target positions with improved selectivity.

Abstract excerpt

The endogenous U1 small nuclear RNA (U1-snRNA) plays a crucial role in splicing initiation through base-pairing to donor splice sites (5'-SSs). Likewise, modified U1s that carry a mutation-adapted 5'-terminal sequence have been demonstrated to rescue exon splicing when this is disrupted by genetic mutations within the 5'-SS. Given the base-pairing flexibility of the endogenous U1, the selectivity of modified U1s...

Topics

• Humans
• Computational Biology
• RNA, Small Nuclear
• RNA Splice Sites
• Binding Sites
• RNA Splicing