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Article

Understanding the stability landscape of LbCas12a by deep analysis of stabilizing mutations and mutation combinations.

Protein science : a publication of the Protein Society - 1 Sept 2025

Dong Jiajun, Wang Shaojie, Xu Wenxue, Xin Jingyao, Liu Jia

Abstract excerpt

Cas12a is one of the most widely used Cas nucleases for genome editing and in vitro diagnosis. A number of engineered Cas12a mutants have been identified with improved activity and stability. However, it remains largely unaddressed how these mutations interact. In a previous study, we used a deep learning model to evolve the stability of Lachnospiraceae bacterium Cas12a (LbCas12a) and obtained about 90 mutants...

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PMID: 40838855
DOI: 10.1002/pro.70280
PMCID: PMC12369400

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