Article
Comparison of the transgenic rodent mutation assay, error corrected next generation duplex sequencing, and the alkaline comet assay to detect dose-related mutations following exposure to N-nitrosodiethylamine.
Mutation research. Genetic toxicology and environmental mutagenesis - 1 Oct 2023
Bercu Joel P, Zhang Shaofei, Sobol Zhanna, Escobar Patricia A, Van Phu, Schuler Maik
Abstract excerpt
N-Nitrosodiethylamine (NDEA), a well-studied N-nitrosamine, was tested in rats to compare the dose-response relationship of three genotoxicity endpoints. Mutant / mutation frequencies were determined using the transgenic rodent (TGR) gene mutation assay and error corrected next generation sequenc...
Topics
- Rats
- Animals
- Male
- Comet Assay
- Diethylnitrosamine
- Rodentia
- Rats, Sprague-Dawley
- Mutation
- Animals, Genetically Modified
- DNA Damage
- High-Throughput Nucleotide Sequencing
- Nitrosamines
- Mutagenicity Tests
- Dose-Response Relationship, Drug