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Pre-Amplification of Cell-Free DNA: Balancing Amplification Errors with Enhanced Sensitivity.

Biomolecules - 17 Jun 2025

Chan Wei Yen, Stewart Ashleigh, Diefenbach Russell J, Gray Elin S, Lee Jenny H, Scolyer Richard A, Long Georgina V, Rizos Helen

Abstract excerpt

Circulating tumour DNA (ctDNA) is a promising biomarker for personalised oncology. However, its clinical utility is limited by detection sensitivity, particularly in early-stage disease. T-Oligo Primed Polymerase Chain Reaction (TOP-PCR) is a commercial amplification approach utilising an efficient "half-adapter" ligation design and a single-primer-based PCR strategy. This study evaluated the clinical value and...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 40563524
DOI: 10.3390/biom15060883
PMCID: PMC12191314

Genes

BRAF
GC
TERT
TP53

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

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