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Fabrication, Evolution, and Mutual Conversion of d-Fucose-Activatable and -Repressible Acetyltransferase upon Mutations.

ACS synthetic biology - 20 Jun 2025

Yanai Yuki, Tsukada Miyu, Kimura Yuki, Umeno Daisuke

Abstract excerpt

The fusion of different proteins can result in the linkage-dependent emergence of molecular switches. We inserted chloramphenicol acetyltransferase (CAT) from Escherichia coli into a loop of a d-fucose-responsive mutant of transcription factor AraC, using linker libraries with various lengths. We found that on-switches tend to emerge when two proteins are fused with linkers long enough to fill the gap of the...

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PMID: 40307007
DOI: 10.1021/acssynbio.4c00169

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CAT

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