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In vitro reconstitution of meiotic DNA double-strand-break formation

19 Feb 2025

Abstract excerpt

The Spo11 complex catalyses the formation of DNA double-strand breaks (DSBs), initiating meiotic recombination—a process that is essential for fertility and genetic diversity1,2. Although the function of Spo11 has been known for 27 years, previous efforts to reconstitute DSB formation in vitro have been unsuccessful. Here we biochemically characterize the mouse SPO11–TOP6BL protein complex, and show that this...

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PMID: 39972125
DOI: 10.1038/s41586-024-08551-1

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