Efficient surface display of single-chain variable fragments against tumor necrosis factor α on engineered probiotic Saccharomyces boulardii and its application in alleviating intestinal inflammation in vivo

Abstract excerpt

Saccharomyces boulardii is a probiotic that can alleviate inflammation in the gut. In this study, a novel surface display system was developed by employing two different single-chain variable fragments (scFvs) against human tumor necrosis factor α as model anti-inflammatory proteins. We first optimized the expression levels of the scFvs by selecting strong constitutive promoters, of which expression cassettes...

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