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The CAG promoter maintains high‐level transgene expression in HEK293 cells

6 Nov 2020

Abstract excerpt

The vast majority of therapeutic recombinant proteins are produced in mammalian cell lines. However, proteins generated in nonhuman cell lines, such as Chinese hamster ovary (CHO) cells, are decorated with human-like glycan structures that differ from those of human cells, and these may induce immunogenic responses in human cells. Human embryonic kidney cells (HEK293F) are also extensively used as hosts for the...

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PMID: 33155423
DOI: 10.1002/2211-5463.13029

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