Engineering of the CMV promoter for controlled expression of recombinant genes in HEK293 cells

doi:10.1002/biot.202200062

Article

Engineering of the CMV promoter for controlled expression of recombinant genes in HEK293 cells

28 Apr 2022

Abstract excerpt

Expression of recombinant genes in HEK293 cells is frequently utilized for production of recombinant proteins and viral vectors. These systems frequently employ the cytomegalovirus (CMV) promoter to drive recombinant gene transcription. However, the mechanistic basis of CMV-mediated transcriptional activation in HEK293 cells is unknown and consequently there are no strategies to engineer CMV for controlled...

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