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Optimization of a novel expression system for recombinant protein production in CHO cells

22 Oct 2024

Abstract excerpt

Chinese hamster ovary (CHO) cells are common mammalian cell lines for expressing recombinant proteins, yet the expression level of recombinant proteins is still hindered. Vector optimization and cell line modification are the key factors to improve the expression of recombinant proteins. In this study, the vector was optimized by adding the regulatory elements Kozak and Leader to the upstream of target gene to...

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PMID: 39438721
DOI: 10.1038/s41598-024-76995-6

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