Article
Mutations to transcription factor MAX allosterically increase DNA selectivity by altering folding and binding pathways.
Nature communications - 14 Jan 2025
Hastings Renee, Aditham Arjun K, DelRosso Nicole, Suzuki Peter H, Fordyce Polly M
Abstract excerpt
Understanding how proteins discriminate between preferred and non-preferred ligands ('selectivity') is essential for predicting biological function and a central goal of protein engineering efforts, yet the biophysical mechanisms underpinning selectivity remain poorly understood. Towards this end, we study how variants of the promiscuous transcription factor (TF) MAX (H. sapiens) alter DNA specificity and...
Read the complete abstract on PubMedTopics
- Humans
- DNA
- Thermodynamics
- Kinetics
- Allosteric Regulation
- Protein Folding
- Protein Binding