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Mutability-Landscape-Guided Engineering of l-Threonine Aldolase Revealing the Prelog Rule in Mediating Diastereoselectivity of C-C Bond Formation.

Angewandte Chemie (International ed. in English) - 9 Jan 2023

Zheng Wenlong, Pu Zhongji, Xiao Lanxin, Xu Gang, Yang Lirong, Yu Haoran, Wu Jianping

Abstract excerpt

l-threonine aldolase (LTA) catalyzes C-C bond synthesis with moderate diastereoselectivity. In this study, with LTA from Cellulosilyticum sp (CpLTA) as an object, a mutability landscape was first constructed by performing saturation mutagenesis at substrate access tunnel amino acids. The combinatorial active-site saturation test/iterative saturation mutation (CAST/ISM) strategy was then used to tune...

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PMID: 36367520
DOI: 10.1002/anie.202213855

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