Article
Production of <scp>d</scp>-Allulose with <scp>d</scp>-Psicose 3-Epimerase Expressed and Displayed on the Surface of <i>Bacillus subtilis</i> Spores
6 Sept 2016
Abstract excerpt
The production of d-allulose is usually conducted via isolated-enzyme or whole-cell biocatalysis reactions. In the present study, an innovative biocatalyst, d-psicose 3-epimerase (DPEase) from Clostridium scindens ATCC 35704, presented on the surface of Bacillus subtilis spores, was applied for d-allulose production. DPEase was fused at the C-terminus of the anchoring protein, CotZ, via a peptide linker, and...
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