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Direct testing of natural twister ribozymes from over a thousand organisms reveals a broad tolerance for structural imperfections.

Nucleic acids research - 11 Dec 2024

McKinley Lauren N, Meyer McCauley O, Sebastian Aswathy, Chang Benjamin K, Messina Kyle J, Albert Istvan, Bevilacqua Philip C

Abstract excerpt

Twister ribozymes are an extensively studied class of nucleolytic RNAs. Thousands of natural twisters have been proposed using sequence homology and structural descriptors. Yet, most of these candidates have not been validated experimentally. To address this gap, we developed Cleavage High-Throughput Assay (CHiTA), a high-throughput pipeline utilizing massively parallel oligonucleotide synthesis and...

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PMID: 39498486
DOI: 10.1093/nar/gkae908
PMCID: PMC11662667

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