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A multi-AS-PCR-coupled CRISPR/Cas12a assay for the detection of ten single-base mutations.

Analytica chimica acta - 1 Sept 2024

Wu Yaozhou, Chang Yanbin, Sun Yingying, Wang Yulin, Li Keke, Lu Zhangping, Liu Qianqian, Wang Fang, Wei Lianhua

Abstract excerpt

Single-nucleotide polymorphism (SNP) detection is critical for diagnosing diseases, and the development of rapid and accurate diagnostic tools is essential for treatment and prevention. Allele-specific polymerase chain reaction (AS-PCR) is widely used for detecting SNPs with multiplexing capabilities, while CRISPR-based technologies provide high sensitivity and specificity in targeting mutation sites through...

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PMID: 39142774
DOI: 10.1016/j.aca.2024.343027

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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