Article
A multi-AS-PCR-coupled CRISPR/Cas12a assay for the detection of ten single-base mutations.
Analytica chimica acta - 1 Sept 2024
Wu Yaozhou, Chang Yanbin, Sun Yingying, Wang Yulin, Li Keke, Lu Zhangping, Liu Qianqian, Wang Fang, Wei Lianhua
Abstract excerpt
Single-nucleotide polymorphism (SNP) detection is critical for diagnosing diseases, and the development of rapid and accurate diagnostic tools is essential for treatment and prevention. Allele-specific polymerase chain reaction (AS-PCR) is widely used for detecting SNPs with multiplexing capabilities, while CRISPR-based technologies provide high sensitivity and specificity in targeting mutation sites through...
Topics
- Polymorphism, Single Nucleotide
- CRISPR-Cas Systems
- Humans
- Polymerase Chain Reaction
- Mutation
- Mycobacterium tuberculosis
- Rifampin
- Limit of Detection
- Multiplex Polymerase Chain Reaction
- Tuberculosis, Multidrug-Resistant