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Article

In situ readout of DNA barcodes and single base edits facilitated by in vitro transcription.

Nature biotechnology - 1 Jan 2020

Askary Amjad, Sanchez-Guardado Luis, Linton James M, Chadly Duncan M, Budde Mark W, Cai Long, Lois Carlos, Elowitz Michael B

Abstract excerpt

Molecular barcoding technologies that uniquely identify single cells are hampered by limitations in barcode measurement. Readout by sequencing does not preserve the spatial organization of cells in tissues, whereas imaging methods preserve spatial structure but are less sensitive to barcode sequence. Here we introduce a system for image-based readout of short (20-base-pair) DNA barcodes. In this system, called...

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Identifiers

PMID: 31740838
DOI: 10.1038/s41587-019-0299-4
PMCID: PMC6954335

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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