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Article

Investigating the effects of a cryptic splice site in the En2 splice acceptor sequence used in the IKMC knockout-first alleles.

Mammalian genome : official journal of the International Mammalian Genome Society - 1 Dec 2024

Nair Prerna, Steel Karen P, Lewis Morag A

Abstract excerpt

Targeted mouse mutants are a common tool used to investigate gene function. The International Knockout Mouse Consortium undertook a large-scale screen of mouse mutants, making use of the knockout-first allele design that contains the En2 splice acceptor sequence coupled to the lacZ reporter gene. Although the knockout-first allele was designed to interfere with splicing and thus disrupt gene function, the En2...

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Identifiers

PMID: 39354111
DOI: 10.1007/s00335-024-10071-2
PMCID: PMC11522132

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