Article
Introduction of Spontaneous Mutations Using Streptomycin as a Method for Lactic Acid Bacteria Breeding.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2024
Namai Fu, Nishiyama Keita, Kitazawa Haruki, Shimosato Takeshi
Abstract excerpt
The chapter presents a technique for inducing spontaneous mutations using antibiotics that target microbial ribosomes and/or RNA polymerase, employed in bacterial breeding. In contrast to UV-based mutagenesis, this method allows control of the mutation sites, specifically targeting the rpsL gene. The outlined methodology introduces spontaneous mutations using streptomycin in Lacticaseibacillus rhamnosus GG ATCC...
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