Article
Mutant sequences in the rpsL gene of Escherichia coli B/r: mechanistic implications for spontaneous and ultraviolet light mutagenesis.
Molecular & general genetics : MGG - 1 Mar 1992
Timms A R, Steingrimsdottir H, Lehmann A R, Bridges B A
Abstract excerpt
Mutants able to grow in the presence of 1.2 mg/ml streptomycin were isolated from Escherichia coli WP2 after exposure to ultraviolet light (UV) or in the absence of any treatment (spontaneous), and from a umuC derivative after exposure to UV and delayed photoreversal. These mutants, characterized as streptomycin resistant (Smr) or dependent (Smd), carry mutations in the rpsL gene. This gene was amplified using...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Drug Resistance, Microbial
- Escherichia coli
- Escherichia coli Proteins
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Polymerase Chain Reaction