Article
Application of In Vitro Transposon Mutagenesis to Erythromycin Strain Improvement in Saccharopolyspora erythraea.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017
Weber J Mark, Reeves Andrew, Cernota William H, Wesley Roy K
Abstract excerpt
Transposon mutagenesis is an invaluable technique in molecular biology for the creation of random mutations that can be easily identified and mapped. However, in the field of microbial strain improvement, transposon mutagenesis has scarcely been used; instead, chemical and physical mutagenic methods have been traditionally favored. Transposons have the advantage of creating single mutations in the genome, making...
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