Article
Genetically engineered rpsL merodiploidy impacts secondary metabolism and antibiotic resistance in Streptomyces.
World journal of microbiology & biotechnology - 17 Mar 2021
Koshla Oksana, Lopatniuk Maria, Borys Oksana, Misaki Yuya, Kravets Volodymyr, Ostash Iryna, Shemediuk Anastasiia, Ochi Kozo, Luzhetskyy Andriy, Fedorenko Victor, Ostash Bohdan
Abstract excerpt
Certain point mutations within gene for ribosomal protein S12, rpsL, are known to dramatically change physiological traits of bacteria, most prominently antibiotic resistance and production of various metabolites. The rpsL mutants are usually searched among spontaneous mutants resistant to aminoglycoside antibiotics, such as streptomycin or paromomycin. The shortcomings of traditional selection are as follows:...
Topics
- Anti-Bacterial Agents
- Bacterial Proteins
- Diploidy
- Drug Resistance, Microbial
- Genetic Engineering
- Mutation
- Plasmids
- Ribosomal Proteins
- Secondary Metabolism
- Streptomyces
- Streptomycin