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Enhanced Thermostability and Catalytic Efficiency of Alginate Lyase Alyw203 by Hydrogen Bond Network Reconstruction.

Marine drugs - 22 Dec 2025

Jiang Chengcheng, Ye Jing-Run, Zhu Tian-Tian, Wang Qin, Ma Yan, Wang Zhi-Peng, Shi Chuan-Yang, Wang Ying, Zhang Shou-Fu, Liu Tian-Hong, Wang Hai-Ying

Abstract excerpt

Alginate lyases are commonly employed for producing alginate oligosaccharides (AOS), but their industrial application is often constrained by low thermal stability and catalytic efficiency. This study engineered mutants of alginate lyase Alyw203 from marine Vibrio based on B-factor values and negative ΔΔG values. The L172V mutant exhibited a 2.43-fold increase in half-life at 40 °C, reduced Km (from 107 to 65...

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Identifiers

PMID: 41590703
DOI: 10.3390/md24010006
PMCID: PMC12843337

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