Article
A Dual-Focus Workflow for Simultaneously Engineering High Activity and Thermal Stability in Methyl Parathion Hydrolase.
Angewandte Chemie (International ed. in English) - 11 Nov 2024
Li Yingnan, Fu Yuzhuang, Chen Xiling, Fan Shilong, Cao Zexing, Xu Fei
Abstract excerpt
Industrial fermentation applications typically require enzymes that exhibit high stability and activity at high temperatures. However, efforts to simultaneously improve these properties are usually limited by a trade-off between stability and activity. This report describes a computational strate...
Topics
- Enzyme Stability
- Phosphoric Monoester Hydrolases
- Molecular Dynamics Simulation
- Protein Engineering
- Methyl Parathion
- Catalytic Domain
- Temperature
- Mutation
- Quantum Theory
- Workflow