Article
Functional analysis of organophosphorus hydrolase variants with high degradation activity towards organophosphate pesticides.
Protein engineering, design & selection : PEDS - 1 Mar 2006
Mee-Hie Cho Catherine, Mulchandani Ashok, Chen Wilfred
Abstract excerpt
Organophosphorus hydrolase (OPH, also known as phosphotriesterase) is a bacterial enzyme that is capable of degrading a wide range of neurotoxic organophosphate nerve agents. Directed evolution has been used to generate one variant (22A11) with up to 25-fold improved hydrolysis of methyl parathion. Surprisingly, this variant also degraded all other substrates (paraoxon, parathion and coumaphos) tested 2- to...
Topics
- Amino Acid Substitution
- Aryldialkylphosphatase
- Binding Sites
- Directed Molecular Evolution
- Escherichia coli
- Genetic Variation
- Hydrogen Bonding
- Hydrolysis
- Kinetics
- Models, Molecular
- Molecular Structure
- Organophosphorus Compounds
- Pesticides
- Plasmids