Article
Rational engineering of a native hyperthermostable lactonase into a broad spectrum phosphotriesterase.
Scientific reports - 1 Dec 2017
Jacquet Pauline, Hiblot Julien, Daudé David, Bergonzi Céline, Gotthard Guillaume, Armstrong Nicholas, Chabrière Eric, Elias Mikael
Abstract excerpt
The redesign of enzyme active sites to alter their function or specificity is a difficult yet appealing challenge. Here we used a structure-based design approach to engineer the lactonase SsoPox from Sulfolobus solfataricus into a phosphotriesterase. The five best variants were characterized and their structure was solved. The most active variant, αsD6 (V27A-Y97W-L228M-W263M) demonstrates a large increase in...
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