Article
A combinatorial multi-site directed mutagenesis solution for improved thermal stability of Lactobacillus plantarum tannase.
Biochemistry and cell biology = Biochimie et biologie cellulaire - 1 Jan 2025
Chevannes T Deandre, St-Jacques Antony D, Loewen Matthew E, Loewen Michele C
Abstract excerpt
This study used a modified flapless (FLT) version of tannase from Lactobacillus plantarum, (LpTan) to explore the effects of "stacking" site mutations predicted by Protein Repair One Stop Shop (PROSS) to increase stability. Four different LpTan structural-state models (including apo, substrate- and product- bound as well as FLT) were comparatively applied, yielding 143 predicted mutations. Of these, eight...
Topics
- Lactiplantibacillus plantarum
- Carboxylic Ester Hydrolases
- Enzyme Stability
- Mutagenesis, Site-Directed
- Temperature
- Models, Molecular
- Mutation