Article
Sensitive and Quantitative Three-Color Protein Imaging in Fission Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with Experimentally-Characterized In Vivo Maturation Kinetics.
PloS one - 1 Jan 2016
Al-Sady Bassem, Greenstein Rachel A, El-Samad Hana J, Braun Sigurd, Madhani Hiten D
Abstract excerpt
Schizosaccharomyces pombe is an outstanding model organism for cell biological investigations, yet the range of useful and well-characterized fluorescent proteins (XFPs) is limited. We generated and characterized three recoded fluorescent proteins for 3-color analysis in S.pombe, Super-folder GFP, monomeric Kusabira Orange 2 and E2Crimson. Upon optimization and expression in S. pombe, the three proteins enabled...
Topics
- Flow Cytometry
- Genetic Vectors
- Genotype
- Image Processing, Computer-Assisted
- Kinetics
- Luminescent Proteins
- Microscopy, Fluorescence
- Protein Folding
- RNA, Messenger
- Real-Time Polymerase Chain Reaction