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Mutation Screening of Dictyostelium Restriction Enzyme-Mediated Integration (REMI) Libraries.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2024

Heslop-Harrison George, Goddard Anthony, Williams Robin S B

Abstract excerpt

Identifying the mechanisms of action of existing and novel drugs is essential for the development of new compounds for therapeutic and commercial use. Here we provide a technique to identify these mechanisms through isolating mutant cell lines that show resistance to drug-induced phenotypes using Dictyostelium discoideum REMI libraries. This approach provides a robust and rapid chemical-genetic screening...

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PMID: 38954208
DOI: 10.1007/978-1-0716-3894-1_15

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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