Article
Generation of multiple knockout mutants using the Cre-loxP system.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2006
Kimmel Alan R, Faix Jan
Abstract excerpt
Dictyostelium discoideum is an exceptionally powerful system by which to study various aspects of modern cell and developmental biology. The completion of the genome sequencing project together with a high-efficiency of targeted gene disruption have enabled researchers to characterize many specific gene functions. However, as a result of many gene products with overlapping functions, there is great need to...
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