Isolation of Dictyostelium discoideum cytokinesis mutants by restriction enzyme-mediated integration of the blasticidin S resistance marker.

doi:10.1006/bbrc.1994.2880

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Isolation of Dictyostelium discoideum cytokinesis mutants by restriction enzyme-mediated integration of the blasticidin S resistance marker.

Biochemical and biophysical research communications - 30 Dec 1994

Adachi H, Hasebe T, Yoshinaga K, Ohta T, Sutoh K

Abstract excerpt

We have developed an improved REMI (restriction enzyme-mediated integration) system for generating mutant Dictyostelium cells quickly and efficiently for systematic screening of cytokinesis mutants. By means of this system, three cytokinesis mutants that grow as giant and multinucleate cells were...

Topics

Animals
Antifungal Agents
Base Sequence
Cell Division
Cloning, Molecular
DNA Restriction Enzymes
DNA, Fungal
DNA, Protozoan
DNA, Recombinant
Dictyostelium
Drug Resistance
Escherichia coli

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Genes, Fungal

Genes, Protozoan

Genetic Markers

Molecular Sequence Data

Mutagenesis

Mutation

Source

Open in PubMed

Identifiers

PMID: 7811269
DOI: 10.1006/bbrc.1994.2880

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-7811269-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Isolation of Dictyostelium discoideum cytokinesis mutants by restriction enzyme-mediated integration of the blasticidin S resistance marker.PMID 7811269Read ↗

Isolation and characterization of a novel cytokinesis-deficient mutant in Dictyostelium discoideum.Semantic proximity Read ↗

The identification of pats1, a novel gene locus required for cytokinesis in Dictyostelium discoideum.Semantic proximity Read ↗

A novel Dictyostelium discoideum gene required for cAMP-dependent cell aggregation.Semantic proximity Read ↗

A novel gene trap method using terminator-REMI and 3' rapid amplification of cDNA ends (RACE) in Dictyostelium.Semantic proximity Read ↗

Identification of proteins involved in cytokinesis of Dictyostelium.Semantic proximity Read ↗

Rapid and efficient genetic engineering of both wild type and axenic strains of Dictyostelium discoideum.Semantic proximity Read ↗

A rapid and efficient method to generate multiple gene disruptions in Dictyostelium discoideum using a single selectable marker and the Cre-loxP system.Semantic proximity Read ↗

Mutation Screening of Dictyostelium Restriction Enzyme-Mediated Integration (REMI) Libraries.Semantic proximity Read ↗

Generation of multiple knockout mutants using the Cre-loxP system.Semantic proximity Read ↗

Gdt2 regulates the transition of Dictyostelium cells from growth to differentiation.Semantic proximity Read ↗

Identification of low frequency knockout mutants in Dictyostelium discoideum created by single or double homologous recombination.Semantic proximity Read ↗

Dictyostelium mutants lacking the cytoskeletal protein coronin are defective in cytokinesis and cell motility.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.