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Article

Effect of Glu235Ser on enzyme performance and in vitro characterization of human β-glucocerebrosidase in a Pichia pastoris.

International journal of biological macromolecules - 1 May 2026

Bayraktar Serpil, Aysan Arzu, Turunen Ossi, Valjakka Jarkko, Binay Barış

Abstract excerpt

Gaucher disease (GD) is the most common lysosomal storage disorder and is caused by inherited deficiency of β-glucocerebrosidase (GCase), resulting in glucocerebroside accumulation in multiple organs. Enzyme replacement therapy (ERT) is associated with complex and costly manufacturing processes, which has motivated the development of scalable expression systems. This study investigates recombinant production of...

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PMID: 41966378
DOI: 10.1016/j.ijbiomac.2026.151928

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