Article
Efficient synthesis of L-malic acid by malic enzyme biocatalysis with CO2 fixation.
Bioresource technology - 1 Jul 2024
Shi Jianping, Fan Yan, Jiang Xinshan, Li Xianglong, Li Shang, Feng Yanbin, Xue Song
Abstract excerpt
The malic enzyme (ME) catalyzes the synthesis of L-malic acid (L-MA) from pyruvic acid and CO2 with NADH as the reverse reaction of L-MA decarboxylation. Carboxylation requires excess pyruvic acid, limiting its application. In this study, it was determined that CO2 was the carboxyl donor by parsing the effects of HCO3- and CO2, which provided a basis for improving the L-MA yield. Moreover, the concentration ratio...
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