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Article

A generic HTS assay for kinase screening: Validation for the isolation of an engineered malate kinase.

PloS one - 1 Jan 2018

Irague Romain, Topham Christopher M, Martineau Nelly, Baylac Audrey, Auriol Clément, Walther Thomas, François Jean-Marie, André Isabelle, Remaud-Siméon Magali

Abstract excerpt

An end-point ADP/NAD+ acid/alkali assay procedure, directly applicable to library screening of any type of ATP-utilising/ADP producing enzyme activity, was implemented. Typically, ADP production is coupled to NAD+ co-enzyme formation by the conventional addition of pyruvate kinase and lactate dehydrogenase. Transformation of enzymatically generated NAD+ into a photometrically active alkali derivative product is...

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Identifiers

PMID: 29462203
DOI: 10.1371/journal.pone.0193036
PMCID: PMC5819781

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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