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Semi-rational design of L-amino acid deaminase for production of pyruvate and D-alanine by Escherichia coli whole-cell biocatalyst.

Amino acids - 1 Sept 2021

Liu Ke, Yu Haoran, Sun Guoyun, Liu Yanfeng, Li Jianghua, Du Guocheng, Lv Xueqin, Liu Long

Abstract excerpt

In our previous study, one-step pyruvate and D-alanine production from D,L-alanine by a whole-cell biocatalyst Escherichia coli expressing L-amino acid deaminase (Pm1) derived from Proteus mirabilis was investigated. However, due to the low catalytic efficiency of Pm1, the pyruvate titer was relatively low. Here, semi-rational design based on site-directed saturation mutagenesis was carried out to improve the...

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PMID: 34417892
DOI: 10.1007/s00726-021-03067-8

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