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A novel type IIb L-asparaginase from Latilactobacillus sakei LK-145: characterization and application.

Archives of microbiology - 18 May 2024

Kato Shiro, Tamura Kazuya, Masuda Yuki, Konishi Morichika, Yamanaka Kazuya, Oikawa Tadao

Abstract excerpt

We succeeded in homogeneously expressing and purifying L-asparaginase from Latilactobacillus sakei LK-145 (Ls-Asn1) and its mutated enzymes C196S, C264S, C290S, C196S/C264S, C196S/C290S, C264S/C290S, and C196S/C264S/C290S-Ls-Asn1. Enzymological studies using purified enzymes revealed that all cysteine residues of Ls-Asn1 were found to affect the catalytic activity of Ls-Asn1 to varying degrees. The mutation of...

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PMID: 38761213
DOI: 10.1007/s00203-024-03979-5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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