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A high efficiency precision genome editing method with CRISPR in iPSCs

30 Apr 2024

Abstract excerpt

The use of genetic engineering to generate point mutations in induced pluripotent stem cells (iPSCs) is essential for studying a specific genetic effect in an isogenic background. We demonstrate that a combination of p53 inhibition and pro-survival small molecules achieves a homologous recombination rate higher than 90% using Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) in human iPSCs. Our...

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PMID: 38688988
DOI: 10.1038/s41598-024-60766-4

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