Article
Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation.
Nature communications - 14 Sept 2020
Li Yanhe, Weng Yuteng, Bai Dandan, Jia Yanping, Liu Yingdong, Zhang Yalin, Kou Xiaochen, Zhao Yanhong, Ruan Jingling, Chen Jiayu, Yin Jiqing, Wang Hong, Teng Xiaoming, Wang Zuolin, Liu Wenqiang, Gao Shaorong
Abstract excerpt
Gene-targeted animal models that are generated by injecting Cas9 and sgRNAs into zygotes are often accompanied by undesired double-strand break (DSB)-induced byproducts and random biallelic targeting due to uncontrollable Cas9 targeting activity. Here, we establish a parental allele-specific gene...
Topics
- Alleles
- Animals
- Base Sequence
- CRISPR-Cas Systems
- Cell Nucleus
- Disease Models, Animal
- Dwarfism
- Embryo Loss
- Female
- Gene Editing
- Gene Targeting
- Genes, Dominant
- Genomic Imprinting
- Heterozygote