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Article

Maternal Supply of Cas9 to Zygotes Facilitates the Efficient Generation of Site-Specific Mutant Mouse Models.

PloS one - 1 Jan 2017

Cebrian-Serrano Alberto, Zha Shijun, Hanssen Lars, Biggs Daniel, Preece Christopher, Davies Benjamin

Abstract excerpt

Genome manipulation in the mouse via microinjection of CRISPR/Cas9 site-specific nucleases has allowed the production time for genetically modified mouse models to be significantly reduced. Successful genome manipulation in the mouse has already been reported using Cas9 supplied by microinjection of a DNA construct, in vitro transcribed mRNA and recombinant protein. Recently the use of transgenic strains of mice...

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Identifiers

PMID: 28081254
DOI: 10.1371/journal.pone.0169887
PMCID: PMC5231326

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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