Protocol for high-quality RNA sequencing, cell surface protein analysis, and genotyping in single cells using TARGET-seq.

Abstract excerpt

Studying the consequences of somatic mutations in pre-malignant and cancerous tissues is challenging due to noise in single-cell transcriptome data and difficulty in identifying the clonal identity of single cells. We optimized TARGET-seq to develop TARGET-seq+, which combines RNA sequencing (RNA-seq), the analysis of cell surface protein expression, and genotyping in single cells with improved sensitivity. We...

Topics

• Single-Cell Analysis
• Membrane Proteins
• Humans
• Sequence Analysis, RNA
• Genotyping Techniques
• RNA-Seq
• Genotype